[Deparaffinization]
(1) Xylene down I: 10 min
(2) Xylene down II: 10 min
(3) 100% ethyl alcohol (= ethanol) down I: 10 min
(4) 100% ethanol down II: 10 min
(5) 90% ethanol down: 10 min
(6) 70% ethanol down: 10 min
(7) Distilled water (= D.W.): 5 min

[Staining]
(1) Carazzi's hematoxylin solution: 15 min
(2) D.W.: 30 sec
(3) 1% HCl-ethanol: 5 times (move a micro slide glass up and down for differentiation, by which excess and easily soluble dye in the tissue is removed after staining)
(4) D.W.: 30 sec
(5) Li₂CO₃ (saturated solution): 30 sec (section coloring turns "blue")
(6) Washing with running tap water: 20 min
(7) Eosin solution: 7 min
(8) D.W.: 30 sec

[Dehydration and Immersion]
(1) 70% ethanol up: 10 min
(2) 90% ethanol up: 2 min
(3) 100% ethanol up I: 3 min
(4) 100% ethanol up II: 5 min
(5) Creosote-xylene up: 5 min
(6) Xylene up I: 5 min
(7) Xylene up II: 10 min
(8) Mount of sections in 6-7 drops of a mixed solution of Canada balsam and xylene with a 24 x 45-55 mm micro cover glass

[Components of each solution]
(1) Carazzi's hematoxylin solution (200 ml): Dissolve hematoxylin (0.2 g) in D.W. (160 ml) by means of heating up to approximately 50 C but not boiling (solution coloring assumes "dark goldenrod"), cool down this solution, and then add NaIO₃ (0.04 g: solution coloring turns "orange red"), powdery AlK(SO₄)₂.12H₂O (10 g: solution coloring turns "indigo"), and glycerin (40 ml) to this solution, in turn (unlike "Delafield's hematoxylin," maturing "Carazzi's hematoxylin" is not necessary)
(2) 1% HCl-ethanol (200 ml): Add 37.2% HCl (2 ml) to 70% ethanol (200 ml)
(3) Saturated Li₂CO₃ solution (200 ml): Dissolve Li₂CO₃ (2 g) in D.W. (200 ml)
(4) Eosin solution (200 ml): Mix 1% eosin solution (50 ml) with 80% ethanol (150 ml), and then add glacial acetic acid (1 ml) to this solution
(5) 1% eosin solution (50 ml): Dissolve water-soluble eosin Y (0.5 g) in D.W. (50 ml), and then add glacial acetic acid (0.1 ml) to this solution
(6) Creosote-xylene (200 ml): Mix creosote (50 ml) with xylene (150 ml)