(1) Fix a dead individual, or an individual sacrificed with anesthesia, in 10% formalin in good standing (more than 1 day). Preservation of the fixed specimen in 70% ethanol is possible.
(2) Subject this specimen to fat removal in acetone (more than 5 days).
(3) Rinse the fat-removed specimen enough with tap water (more than 1 day).
(4) Strip off the skin of this specimen, using tools such as tweezers.
(5) Keep the stripped specimen in 2-4% KOH solution, till the skeleton is faintly visible through the muscle (1-3 weeks). This manipulation takes too much time when exposed to low temperatures.
(6) Keep this specimen in a mixed solution of 2-4% KOH solution (6 vols.) and the "staining solution*" (1 vol.), for staining (1 day).
(7) Keep this specimen in 2-4% KOH solution, for differentiation (2-3 days).
(8) Keep this specimen in a mixed solution of 2-4% KOH solution (3 vols.) and glycerin (1 vol.), till the skeleton is clearly visible through the muscle (1-3 weeks).
(9) Keep this specimen in a mixed solution of 2-4% KOH solution (1 vol.) and glycerin (3 vols.), till the specimen sinks to the bottom of the vessel (2-3 days).
(10) Preserve this specimen in glycerin with a small amount (to a pick of earwax) of powdery thymol (i.e., antiseptic).

[Components of the staining solution (150 ml)]
*Dissolve alizarinsulfonate (100 mg) in 1% chloral hydrate solution (120 ml), and then add glycerin (20 ml) and glacial acetic acid (10 ml), in turn, to this solution.